【SUMMARY】
HAV is a positive RNA virus, a unique member of picornavirdae. Its transmission depends primarily on serial transmission from person to person by the fecal-oral route. Although hepatitis A is not ordinarily a sexually transmitted disease, the infection rate is high among male homosexuals, as result of oral-anal contact .

The presence of specific anti-HAV IgM in blood samples suggests acute or recent HAV infection 4-6 . The IgM antibody rapidly increases in titer over a period of 4-6 weeks post infection, and then declines to non-detectable levels within 3 to 6 months in most patients .
The HAV IgM Rapid Test Cassette is to be used to detect anti-HAV IgM in less than 20 minutes by untrained or minimally skilled personnel, without cumbersome laboratory equipment.

【DIRECTIONS FOR USE】
Allow the test cassette, specimen, buffer and/or controls to reach room temperature (15-30°C) prior to testing.
1. Sample Dilution
Use micropipette to add 50mL specimen into the sample dilution tube. Screw the lid tightly and shake it for 10 seconds to ensure the solution could be well mixed. Use the diluted sample as specimen for testing. See instruction below.

 

2. Testing Procedures
Remove the test cassette from sealed pouch and use it within one hour. Best results will be obtained if the assay is performed immediately after opening foil pouch.
Hold the dropper vertically, draw the diluted specimen from sample dilution bottle upto the fill line marked on the dropper as shown in illustration below (approx.5 μL), transfer the diluted specimen to the sample area (S) which has been marked on the test cassette. Or use micropipette to add 5L diluted specimen into the sample area (S) which has been marked.
Add 2 drops of buffer (approx. 80 μL) into the buffer well (B) marked on the test cassette, start the timer. See illustration below.
Wait for the colored line(s) to appear .  Read the result at 20 minutes, do not interpret the result after 30 minutes.

 

Cat. No.	Product Description	Specimen	Format	Kit Size	Cut-Off	Status
IHA-302	HAV IgM Rapid Test Cassette	S / P	Cassette	25 T	See Insert	CE