【SUMMARY】
HIV (Human Immunodeficiency Virus) is the etiologic agent of Acquired Immune Deficiency Syndrome (AIDS). The virus is surrounded by a lipid envelope that is derived from the host cell membrane. Several viral glycoproteins are on the envelope. Each virus contains two copies of positive-sense genomic RNAs. HIV-1 has been isolated from patients with AIDS and AIDS-related complex, and from healthy people with high potential risk for developing AIDS. 1 HIV-1 consists of Subtype M and Subtype O. Highly divergent strains of HIV-1 were first recognized in 1990 and grouped provisionally as Subtype O as this variation has similar glycoprotein markers to HIV-1 but a slight variation to the protein marker. Although rarely compared to HIV-1 and HIV-2, infections caused by Subtype O have so far been identified in Africa (Cameroon), France and Germany. HIV-2 has been isolated from West African AIDS patients and from seropositive asymptomatic individuals. 2 HIV-1, HIV-2, and Subtype O all elicit immune responses. 3 Detection of HIV antibodies in whole blood is the most efficient and common way to determine whether an individual has been exposed to HIV and to screen blood and blood products for HIV. 4 Despite the differences in their biological characters, serological activities and genome sequences, HIV-1, HIV-2, and Subtype O show strong antigenic cross-reactivity. 5,6 The HIV 1.2.O Rapid Test Cassette (whole blood) is a rapid test to qualitatively detect the presence of antibodies to HIV type 1, type 2, and/or Subtype O in whole blood specimen.

【DIRECTIONS FOR USE】
Allow the test pack to equilibrate to room temperature (15-30°C) prior to testing.
1. Open the small part of the pouch, remove the test cassette and place it on a clean and level surface. Run the testing within one hour and best results will be obtained if the test is performed immediately after opening the foil pouch.
2. Open the large part of the pouch, remove the transfer pipette, buffer vial, sterile lancet and alcohol swab, place them close to the test cassette. Twist off the tab of the buffer vial without squeezing and keep it ready for testing.
3. Use the provided alcohol swab to clean the fingertip of the middle finger as the puncture site.
4. Carefully pull off the sterile lancet cap. Push the sterile lancet firmly into the fingertip of the middle finger. Do not use the first drop of blood. To increase blood flow, use the thumb and forefinger to gently apply pressure around the puncture site.
5. Hold the disposable transfer pipette vertically and aspirate the blood drop from puncture site. Dispense 2 drops of blood (approx. 50μL) from the transfer pipette into the specimen well (S) on the test cassette, and then add 2 drops of buffer (approx. 80μL). Avoid touching the transfer pipette directly to the fingertip. Start the timer.
6. Wait for the colored line(s) to appear. Read results at 10 minutes. Do not interpret results after 20 minutes.

Cat. No.	Product Description	Specimen	Format	Kit Size	Cut-Off	Status
IHI-T402S	HIV 1.2.O Rapid Test Cassette Single Use Kit	WB	Cassette	1 T	See Insert	Non-CE