Alpha-Pyrrolidinovalerophenone (also known as α-PVP, A-PVP, alpha-PVP, and Flakka) is a synthetic stimulant substance of the cathinone and pyrrolidine chemical classes. α-PVP may be quantified in blood, plasma or urine to confirm a diagnosis of poisoning in hospitalized patients or to provide evidence in a medicolegal death investigation. It generally comes in the form of either a crystalline powder or crystallized shards which users can ingest to produce powerful but short-lived euphoric stimulant effects which are comparable to those of methamphetamine and cocaine when insufflated or vaporized. α-PVP has been reported to be the cause, or a significant contributory cause of death in suicides and overdoses caused by combinations of drugs. It has also been linked to at least one death where it was combined with pentedrone and caused heart failure.

The α-PVP Rapid Test Cassette (Oral Fluid) is a rapid oral fluid screening test that can be performed without the use of an instrument. The test utilizes a monoclonal antibody to selectively detect elevated levels of alpha-Pyrrolidinovalerophenone in human oral fluid. The α-PVP Rapid Test Cassette/ Midstream(Oral Fluid) yields a positive result when alpha-Pyrrolidinovalerophenone in oral fluid exceeds 300ng/ml.

 

 

【DIRECTIONS FOR CASSETTE USE】

Allow the test cassette, specimen, and/or controls to reach room temperature (15-30ºC)

prior to testing. Instruct the donor to not place anything in the mouth including food, drink, gum or tobacco products for at least 10 minutes prior to collection.

1. Remove the test cassette from the sealed pouch and use it within one hour.

2. Remove the collector from the sealed pouch and insert the sponge end of the collector into the mouth. Actively swab the inside of the mouth and tongue to collect oral fluid for a total of

3 minutes until the sponge becomes fully saturated. Gentle pressing the sponge between the tongue and teeth will assist saturation. No hard spots should be felt on the sponge when saturated.

3. Remove the collector from the mouth. Place saturated oral fluid collector into chamber and press sponge fully against the strainer to collect oral fluid. Discard the collector. Snap the cap shut on the collection chamber.

4. Place the test cassette on a clean and level surface. Unscrew cap cover from the collection chamber. Invert the collection chamber and transfer 3 drops of oral fluid (approximately 120 µL) into specimen well of the test cassette and start the timer. Avoid trapping air bubbles in the specimen well. Place screw cap on the collection chamber. See illustration below.

5. Wait for the colored line(s) to appear. Read result at 10 minutes. Do not read results after 1 hour.

6. Apply the security seal over screw cap and send to the laboratory for confirmation if necessary.