Methadone is an unusual drug in that its primary urinary metabolites (EDDP and EMDP) are cyclic in structure, making them very difficult to detect using immunoassays targeted to the native compound.1Exacerbating this problem. there is a subsection of the population classified as “extensive metabolizers” of methadone. In these individuals, a whole blood/serum/plasma specimen may not contain enough parent methadone to yield a positive drug screen even if the individual is in compliance with their methadone maintenance. EDDP represents a better whole blood/serum/plasma marker for methadone maintenance than unmetabolized methadone.

 

【DIRECTIONS FOR USE】

Allow the test, specimen, buffer and/or controls to reach room temperature (15-30°C) prior to testing.

For serum or plasma specimen:

1. Bring the pouch to room temperature (15-30℃) before opening it. Remove the cassette from the sealed pouch and use it within one hour.

2. Place the cassette on a clean and level surface. Hold the dropper vertically and transfer 1 full drop of serum or plasma (approximately 40 μl), then add 2 drops of buffer (approximately 80 μl) to the specimen well of the cassette, and then start the timer. Avoid trapping air bubbles in the specimen well. See illustration below.

3. Wait for the colored line(s) to appear. Read the result at 5 minutes. Do not interpret the result after 10 minutes.

 

 

For whole blood specimen:

1. Bring the pouch to room temperature before opening it. Remove the test cassette from the sealed pouch and use it as soon as possible.

2. Place the cassette on a clean and level surface.

For Venipuncture Whole Blood specimen:

 Hold the dropper vertically and transfer 1 drop of whole blood (approximately 40 μl) to the specimen well, then add 2 drops of buffer (approximately 80 μl), and start the timer. See illustration below.

For Fingerstick Whole Blood specimen:

 To use a capillary tube: Fill the capillary tube and transfer approximately 40 μl of fingerstick whole blood specimen to the specimen area of test cassette, then add 2 drops of buffer(approximately 80 μl) and start the timer. See illustration below.

3. Wait for the colored line(s) to appear. Read results at 5 minutes. Do not interpret the result after 10 minutes.

 

 

 

 

 

 

 

Cat. No.	Product Description	Specimen	Format	Kit Size	Cut-Off	Status
DED-402	EDDP Rapid Test Cassette	WB/S/P	Cassette	40 T	50 ng/mL	CE